Consistent with those studies, we found that a glycolysis signature composed of four glycolytic genes (NUP205, NUPL2, PFKFB1 and PKM) can predict the survival of patients with bladder cancer accurately in the TCGA cohort as well as in two independent GEO cohorts, suggesting its favorable performance for prediction. This evidence concerns the gene PFKFB1 and urinary bladder cancer.