Patient-derived melanoma cell lines used in the present study exhibited a constitutive activity of NF-κB that is in agreement with our previous studies reporting substantial levels of phosphorylated p65 in cells harboring mutations in either BRAF or RAS [22,23], and indicating that NF-κB activity is independent of extracellular growth factor stimuli [56]. This evidence concerns the gene NFKB1 and melanoma.