To further confirm that the protease activity of NS2B3 is involved in the specific cleavage/degradation of Nup98, Nup153, and TPR during ZIKV infection, the Nups integrity was analyzed by Western blot in Huh-7 cells transfected with plasmids containing the active (ZIKV NS2-NS3B3 WT) or inactive (ZIKV NS2B3-S135A mut) sequence of the ZIKV protease. This evidence concerns the gene TPR and Zika virus infectious disease.