Furthermore, results from transwell and western blot assays elucidated that FOXK1 offsets the obstructive effects of TMPO‐AS1 deficiency on invasion, migration, and EMT process of HCC cells(Figure 4F, Figure S4E‐G).Overall, TMPO‐AS1 promoted HCC by regulating the miR‐329‐3p/FOXK1 axis. This evidence concerns the gene FOXK1 and hepatocellular carcinoma.