The results of western blotting showed that HOTAIR interference increased E-cadherin expression and decreased Vimentin expression compared to control (Figure 3(a)).Moreover, we found that the green fluorescence(E-cadherin) increases and the red fluorescence(Vimtein) decreases after HCC cells were transfected with si-HOTAIR(Figure 3(b)). This evidence concerns the gene VIM and hepatocellular carcinoma.