TWIST1 and neoplasm: More detailed gene expression analysis revealed that, in compliance with the epithelial-mesenchymal phenotype, the mixed-type organoid cultures showed upregulation of several EMT-related genes (e.g., Vim, Twist1, and Zeb1/2) compared with the original clone, while non-mesenchymal tumor derivatives largely lacked the expression of such genes (Fig. 6g).