HNRNPH2 and glioblastoma: To demonstrate a functional role for hnRNP H/F in translational regulation, we transfected U87 or LN18 GBM cells with hnRNP H and/or hnRNP F specific or control siRNAs for 48 h, followed by quantification of global protein synthesis rates by pulse-labeling with puromycin and immunoblotting using an anti-puromycin antibody (i.e., SUnSET assay).