DHX36 and glioblastoma: To investigate the possibility that hnRNP H/F and DHX36 cooperate to regulate RG4-dependent translation, we first performed co-immunoprecipitation assays using total (TE) or cytoplasmic (CE) extracts from U87 (Fig. 4a) or U251 (Supplementary Fig. 9a) GBM cells, in the presence of RNase and DNase to exclude nucleic acid-mediated interactions.