To further explore the translational regulation mediated by hnRNP H/F bound to RG4 forming sequences, we transfected GBM U87 ((Fig. 3g) or U251, Supplementary Fig. 8e) cells with in vitro-transcribed USP1 RNA reporters containing the RG4 sequence WT (USP1 RG4 WT), 7dG-modified (USP1 RG4 7dG) or mutated (USP1 RG4 Mut). The gene discussed is UNC119; the disease is glioblastoma.