To validate the primary screen, genes of interest were selected from following categories: (i) fitness factors for single-species infections (flagella [encoded by fliC]), (ii) core fitness factors for both single-species infections and polymicrobial infections (twin arginine translocation [tatC], an ATP-dependent protease [clpP], d-alanine–d-alanine ligase [ddlA], and type 3 secretion [T3S] [yscI, yscC, and sopB]), and (iii) fitness factors for polymicrobial infection (type VI secretion [impJ]). This evidence concerns the gene CLPP and infection.