The factors clearly associated with OS or RFS of gallbladder carcinomas were age, preoperative serum level of CA19–9, tumor stage, T category of tumor stage, lymph node metastasis, distant metastasis, lymphovascular invasion, histologic type, histologic grade, and the expression of nuclear FAM83H (OS; p <  0.001, RFS; p <  0.001), cytoplasmic FAM83H (OS; p <  0.001, RFS; p = 0.001), nuclear ZNF16 (OS; p <  0.001, RFS; p <  0.001), and cytoplasmic ZNF16 (OS; p = 0.035, RFS; p = 0.068) (Table 2). This evidence concerns the gene ZNF16 and neoplasm.