Multivariate analysis was performed with the factors clearly associated with OS or RFS; age, preoperative serum level of CA19–9, tumor stage, T category of tumor stage, lymph node metastasis, distant metastasis, lymphovascular invasion, histologic type, histologic grade, and the expression of nuclear FAM83H, cytoplasmic FAM83H, nuclear ZNF16, and cytoplasmic ZNF16. This evidence concerns the gene ZNF16 and metastatic malignant neoplasm in the lymph nodes.