We first determined the Dox concentration required to induce RUNX1-ETO expression in hematopoietic progenitors to levels that mimicked the balanced ratio of RUNX1-ETO:RUNX1 expression observed in AML patients (Figure S1E), suggesting 3–5 ng/mL Dox as the most appropriate concentrations. Here, RUNX1T1 is linked to acute myeloid leukemia.