Although previous studies suggest that components of the NOR are redistributed after demyelination in the PNS (England et al., 1990) and in MS lesions (Coman et al., 2006; Craner et al., 2004; Dupree et al., 2004), our studies reveal that βIV-spectrin, which forms a complex with Ankyrin-G to link voltage-gated sodium channels to the actin cytoskeleton at NORs (Susuki et al., 2016), remains clustered (without flanking myelin sheaths) with continued administration of cuprizone for up to six weeks (Figure 7D,F), suggesting that nodal guideposts remain for many weeks after demyelination. Here, ANK3 is linked to myeloid sarcoma.