In order to analyze the relationship between miR-34c-5p and NOTCH1 and how this relationship affects the development of NPC, we constructed three vectors, miR-34c-5p mimics, miR-34c-5p inhibitor, and NOTCH1 siRNA, to change the expression of miR-34c-5p and NOTCH1 in NPC. The gene discussed is NOTCH1; the disease is nasopharyngeal carcinoma.