As shown in Figure 4, positive AFP (>=200) was found in 29.4%, 42.8%, and 57.7% hepatocellular carcinoma (HCC) patients with size less than 3 cm (Figure 4A), between 3 and 5 cm (Figure 4B), and greater than 5 cm (Figure 4C), respectively. This evidence concerns the gene AFP and hepatocellular carcinoma.