To further investigate the effects of KLC4 in regulating the fate of lung cancer cells treated with anticancer drugs, the gene was silenced via RNA interference using specific siRNA targeting KLC4. As illustrated in Fig. 2c and Supplementary Fig. 1c, KLC4 was successfully knocked down in R-H460 and A549 cells after transfection with the KLC4 siRNA. This evidence concerns the gene KLC4 and lung cancer.