To evaluate the effects on the amyloid phenotype of AD mice, from the genetic modification of the endogenous muTNF-α gene, we used the 5XFAD transgenic mice26 which bear five familial AD mutations, three for the APP and two for the presenilin 1 gene, and develop an amyloid phenotype with amyloid deposition at 2 months of age and robust glial activation. The gene discussed is PSEN1; the disease is Alzheimer disease.