Moreover, in bladder cancer, nucleolar and spindle associated protein 1 (NUSAP1) was upregulated, and its expression was closely related to the poor prognosis of patients, which was also demonstrated to regulate EMT via the TGF-β signaling pathway, as well as p-Smad2/3 and vimentin expression [34]. The gene discussed is TGFB1; the disease is urinary bladder carcinoma.