Confirmation of the array data by qRT-PCR analysis revealed that the mRNA transcript levels of CCL20 and IL8 in HEK293 and a panel of CRC cells were further augmented by the overexpression of WT-PRMT5 under IL-1β-stimulating conditions whereas these transcripts were significantly attenuated by S15A overexpression (Figure 2C). The gene discussed is IL1B; the disease is colorectal carcinoma.