FASN over-expression in PDX model and SW480 colon cancer cells led to an upsurge in the expression of SphK2 but also of SphK1 by activating ERK1/2, while pharmacological inhibition of FASN specifically suppressed the expression of SphK2 in primary colorectal cancer cells and PDX model of metastatic colorectal cancer. Here, MAPK3 is linked to malignant colon neoplasm.