We used Western blotting to assess the expression of TXNIP and NOX4 and changes in TXNIP/NLRP3; the results show that treatment with PU reduces the expression of NOX4, and the expression of TXNIP and NLRP3 also decreased, which is in line with our expectation about the relationship between NOX4 and the TXNIP/NLRP3 pathway and the regulatory role of NOX4 in diabetic nephropathy. The gene discussed is NLRP3; the disease is diabetic kidney disease.