To investigate the relationship between genetic alterations and the CHRNB4 signature for smoking HNSCC patients, we identified 246 genes with significantly different copy number alterations (CNAs) between 87 CHRNB4-high and 88 CHRNB4-low smoking patients by using Fisher’s exact test (only 83 of 87 CHRNB4-high patients’ copy number data were obtained from the database, details are found in the Materials and Methods section and Equation (2)). Here, CHRNB4 is linked to head and neck squamous cell carcinoma.