In the present study, we tested the effect of two different routes of ASC-exosomes administration (i.v. or i.n.)on SOD1(G93A) murine model of ALS, and demonstrated that repeated administration improved the motor performance; protected lumbar motoneurons, the neuromuscular junction, and muscle; and decreased the glial cells activation in the exosomes-treated animals. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.