To demonstrate that SPON1 improves the cognition of AD model mice, we injected lentiviral human SPON1 or mKate2 (MOCK control) into both the hippocampus and entorhinal cortex (EC) of 5xFAD mice (three months old) at an early stage of AD pathological development (Figure 4A,B). The gene discussed is SPON1; the disease is Alzheimer disease.