Mechanism studies reported that altholactone inhibited the growth of human bladder cancer T24 cells (IC50 = 43.5 μM) by inducing apoptosis [35] and induced DU145 cell death (IC50 = 38.5 μM), through inhibition of NF-κB and STAT3 activity [34]. The gene discussed is NFKB1; the disease is urinary bladder cancer.