Serum TNF-α levels in insulin-treated, vitamin D-treated, and combined insulin and vitamin D-treated groups were significantly lower than that in DN group (38.06 ± 2.44, 41.58 ± 1.96, 32.1 ± 2.52 pg/mL, respectively; P < 0.05) and significantly higher than that in control group (P < 0.05). The gene discussed is TNF; the disease is liver dysplastic nodule.