In the present study, under TDH treatment, the expression of p-p70S6K and β-catenin was significantly reduced in LoVo cells; however, the expression of p-p70S6K and β-catenin was upregulated, apoptosis decreased, and cell migration increased after using mTOR and Wnt signaling pathway agonists; the result suggested that TDH may exert antitumor activity through inhibiting the PI3K/AKT/mTOR and Wnt/β-catenin signaling pathways in the colon cancer. This evidence concerns the gene TDH and malignant colon neoplasm.