The Human Protein Atlas database (https://www.proteinatlas.org/) and GeneCards database (https://www.genecards.org/) were then employed to analyze the subcellular localization of ZNF139. As clearly shown in Figure 2A, ZNF139 was mainly localized to the nuclear bodies and its additional location was nucleoplasm and mitochondria in human epidermal cancer A-431 cells, human osteosarcoma U-2 OS cells, and human glioma U-251 MG cells. This evidence concerns the gene ZKSCAN1 and osteosarcoma.