To further examine the impact of T-DM1 on cytokine-mediated bystander killing in our panel of breast and ovarian cancer cell lines, we treated JIMT1, SKOV3, T47D and MCF7 cells with T-DM1, colchicine, trastuzumab or vehicle, in the presence or absence of 100 μg/ml TNFα. This evidence concerns the gene TNF and ovarian cancer.