To further confirm these data the mRNA expression of Spry1 was analyzed by using the Human Cancer Metastasis Database (HCMDB) (http://hcmdb.i-sanger.com/index)32, and the results of GSE15605 (Exp_00028) and GSE7553 (Exp_00365 and Exp_00366) datasets demonstrated that the mRNA levels of Spry1 were significantly up-regulated in metastatic CM compared with primary lesions (p value <0.01) (Fig. 1b). The gene discussed is SPRY1; the disease is cancer.