ZFAS1 and colorectal carcinoma: RT-qPCR analyses indicated that the inhibition of ZFAS1 expression positively affected NOP58 expression in both HCT116 and SW620 cells, and similarly, NOP58 protein expression levels were also decreased after ZFAS1 inhibition in HCT116 and SW620 CRC cells as determined by WB and IF assays (Fig. 4d, e, Fig. S4 b, c).