To evaluate the molecular mechanism of ZFAS1 interaction with NOP58 in the context of CRC in vivo, we first established xenografts in BALB/c nude mice by inoculating HCT116 cells that were stably co-transfected with ZFAS1 and NOP58-WT, NOP58-Mut vector, or NC controls within the same mice at their right armpits (Fig. 7a). Here, ZFAS1 is linked to colorectal carcinoma.