To further verify the impact of ZFAS1 on the biological mechanisms of CRC, we used CCK8 assays to determine that overexpression of ZFAS1 significantly enhanced the proliferation of HCT116 and SW620 cells, while knockdown of ZFAS1 suppressed the proliferative ability of these two cell lines (Fig. 4a). The gene discussed is ZFAS1; the disease is colorectal carcinoma.