SIRT1 levels were decreased in the livers of NAFLD patients, SIRT1 knockdown in human induced pluripotent stem cells (iPSCs)-derived hepatocytes increased fatty acid biosynthesis, and the implantation of these cells in decellularized rat livers resulted in steatosis and inflammation similar to that in human fatty livers [72]. This evidence concerns the gene SIRT1 and metabolic dysfunction-associated steatotic liver disease.