Our network analysis revealed that, among 17 cancer-related genes, MUTYH and PRKAR1A indirectly interact with MUS81, via MSH2 or CHECK2, while ERRC3 directly interacts with MUS81. The top significant pathways with related to cancer and MUS81 were DNA replication, nucleotide excision repair, and lagging strand synthesis. This evidence concerns the gene MSH2 and cancer.