In addition to TIM-3, expression of another immune suppressive receptor, lymphocyte activation gene-3 (LAG-3), is also increased in immune infiltrates of HCC tissues, suggesting that PD-1, TIM-3, and LAG-3 may cooperate and are implicated in inducing anti-tumor immune tolerance [14,47]. This evidence concerns the gene PDCD1 and hepatocellular carcinoma.