When analyzing a monogenetic ALS cell line, namely the SOD-1-mutant cell line (p.R115G), we saw a significant decrease in the number of MNs relative to total cells (MN/Hoechst) when comparing ALS- or control-CSF-treatment to non-treated cells (10.59% vs. 38.24%, p < 0.05; 15.59% vs. 38.24%, p < 0.05; one-way ANOVA with Tukey post-hoc test) (Figure 2b). This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.