The results were similar to those of in vitro studies showing that combined administration of CO and RF in HFD-induced obese mice promoted the downregulation of adipogenesis-associated genes Cebpa, Fabp4, Pparg and Srebp1 compared to those in HFD-induced-obesity mice (Figure 4). The gene discussed is SREBF1; the disease is obesity due to melanocortin 4 receptor deficiency.