We have evaluated the method by measuring the binding of the Wnt signaling inhibitors human Dickkopf1 (DKK1) and Dickkopf2 (DKK2) to the Wnt co-receptor lipoprotein-receptor related protein 6 (LRP6) in human lung cancer (NCI-H1703) and human embryonic kidney (HEK293T) cells (Mao et al., 2001; Cruciat and Niehrs, 2013). Here, DKK2 is linked to lung cancer.