GPT and metabolic dysfunction-associated steatotic liver disease: Insulin resistance can lead to excessive accumulation of lipids and inflammation in the liver, which are key contributors to NAFLD.[1, 3, 4] After the HFD feeding period, the increased liver size and weight, the ratio of liver weight to body weight, and levels of plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic cholesterol, and triglyceride were attenuated in TMLKO mice.