The reduction of cell growth, colony formation, migration, and invasion induced by KD of Sp4 in HCT‐116 and SW480 CRC cells could be efficiently rescued to the control level after the reexpression of L‐Sp4 but not S‐Sp4 (Figure 7C–E), indicating that the L‐Sp4 isoform exerts oncogenic functions, while the S‐Sp4 isoform has no oncogenic functions. Here, SP4 is linked to colorectal carcinoma.