In the present study, we tried to assess the expression of miR-34a, miR-1827, miR-449a, and miR-106b, which were selected by bioinformatics approaches, and three principal genes of Notch signaling pathway, i.e. NOTCH1, c-Myc, and CCND1, in a T-ALL cell line and clinical samples. This evidence concerns the gene CCND1 and acute lymphoblastic leukemia.