Therefore, the expressions of Ki67 and PCNA were detected by immunohistochemistry, generally used clinically, in this study (40), and our result confirm that after the combined icotinib and pemetrexed treatment, the PIs of PCNA and Ki67 in the NSCLC rats injected with anti-miR-22 were appreciably lower than other resting groups, which is also consistent with our findings by using Western blotting. This evidence concerns the gene MKI67 and non-small cell lung carcinoma.