To determine the independent impact of Raptor and hyperglycemia on β-cell identity and function, we implanted slow-release insulin pellet on 4-week-old βRapKOGFP mice (the age when fasting blood glucose levels started to rise) for 4 weeks and kept the serum blood glucose at normal levels in mutant rodents (Fig. 2a). Here, RPTOR is linked to Hyperglycemia.