Western blotting indicated that SAV1 protein levels were 5.7 times higher in tissues from the lycorine-treated mice, and YAP protein levels were 4.2 times lower in the tumor tissues from the lycorine-treated mice compared with controls (Fig. 2f, g, h); whereas RT-PCR (Fig. 2i) and quantitative PCR (qPCR) (Supplementary Fig. S2e) found no difference in mRNA levels between these two groups. This evidence concerns the gene SAV1 and neoplasm.