Figure 1B indicates the scoring distribution of AMPKα1 and AMPKα2 among the patient samples. Each sample received a score of 4, 5, or 6 for both AMPKα1 and AMPKα2, indicating strong and/or widespread expression in each tumor. Next, cellular localization of AMPKα1 and AMPKα2 was compared in these patient samples. As shown in Figure 1C, AMPKα1 was expressed only in the cytoplasm, while AMPKα2 was found in both the cytoplasm and the nucleus. Immunoblotting was then used to study the expression of both isoforms in a panel of TNBC cell lines (Figure 1D). This evidence concerns the gene PRKAA1 and neoplasm.