Inversely, CD4 T cells, CD8 T cells, and NK cells were showed a reduction trend in heat stroke lung (Figure 9), among these, the estimate enrichment scores of CD4+ naïve T-cells (0.57-fold, P value = 0.0066) (Figure 9E), CD8+ Tcm (0.32-fold, P value = 0.035) (Figure 9F), CD8+ Tem (0.32-fold, P value = 0.0233) (Figure 9G), NK cells (0.36-fold, P value = 0.0092) (Figure 9H) and Tregs (0.56-fold, P value = 0.0168) (Figure 9I) were reduced with statistical significance. This evidence concerns the gene CD4 and Heat Stroke.