FOXP4 and hepatocellular carcinoma: Luciferase assay showed the relative luciferase activity in cells co-transfected with FOXP4 luciferase reporter and miR-4651 mimics was significantly decreased approximately 50% compared with the controls or wild-types; while cells were co-transfected with FOXP4-mutant luciferase plasmid and miR-4651 mimics, the relative luciferase activity was no obviously changes compared with the controls or wildtypes in both HCC cell lines (Figure 3B,C).