Two different cell types (LN18 glioma cells genetically modified by lentiviral vector transduction to overexpress the R132H mutant form of isocitrate dehydrogenase 1 (IDH1) and untransduced IDH1 wild-type cells) were grown to 90% confluency at two different glucose concentrations (5 and 25 mM glucose) providing four biological groups (quality control (QC) samples were comprised of an equal-volume mixture of all four experimental group samples). Here, IDH1 is linked to central nervous system cancer.