Subcutaneous infusion of angiotensin II (600 ng/kg/min) in combination with 1% NaCl in the drinking water was initiated at the age of 12 weeks in male C57BL/6 N mice and continued for 4 weeks in reference hypertension mice and for 4 weeks and nine days in buffer hypertension mice and MDCO-216 hypertension mice. The gene discussed is AGT; the disease is hypertensive disorder.