Our results showed that P16, RASSF1A, RPRM, and RUNX3 promoters were methylated in 41.7, 33.3, 66.7, and 58.3% of the GC patients (I–IV), and in 15.9, 0.0, 6.8 and 4.5% of the healthy controls, respectively (p<0.001). This evidence concerns the gene RUNX3 and gastric cancer.