For example, in addition to GC, aberrant methylation of P16 and RASSF1A in serum of breast cancer patients 34, hypermethylation of RASSF1A in hepatocellular carcinoma tissues 35, and hypermethylation of RUNX3 in serum of colorectal cancer patients have also been reported 36. This evidence concerns the gene RUNX3 and breast carcinoma.