These results are accordance with the data in GEPIA (Gene Expression Profiling Interactive Analysis) and TCGA (The Cancer Genome Atlas) that Gli1 expression was higher in clinical stage (2/3/4) compared with clinical stage (1) (P < 0.001), and was not correlated with lymph node metastasis (Supplemental Figure). Here, GLI1 is linked to metastatic malignant neoplasm in the lymph nodes.