However, both M13HS-2 and M13HS-8 hybrids [29] and M13MDA435–1 and M13MDA435–3 hybrids lack E-Cadherin expression (unpublished data), but were able to form mammospheres suggesting that the capability of breast cancer cell lines to form mammospheres does not only depend on E-Cadherin expression. The gene discussed is CDH1; the disease is breast carcinoma.