On the basis of the evidence of a dysregulation in Nrf2 and AMPK pathway genes in liver tissue of patients with NAFLD by employing transcriptomic and RNA sequence analyses provided by the present study, we evaluated the effects of Lip-C6 on the AMPK/Nrf2 pathway in primary human HSC, key cellular effectors in the progression from NAFLD to NASH. This evidence concerns the gene PRKAB1 and metabolic dysfunction-associated steatotic liver disease.